Article abstract

International Journal of Biotechnology and Food Science

Research Article | Published November 2017 | Volume 5, Issue 4, pp. 56-62

 

Effects of live Lactobacillus paracasei NFRI 7415 on the intestinal immune system and intestinal microflora of mice

 

 

 

 

 

 Noriko Komatsuzaki1*

 Ayako Aoki-Yoshida2

 Jun Shima3

 

  Email Author

 

   1.    Department of Human Nutrition, Seitoku University, 550 Iwase, Matsudo, Chiba 271-8555, Japan.

 

  2.    Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

 

  3.    Faculty of Agriculture, Ryukoku University, 1-5 Yokotani, Seta Oe-cho, Otsu, Shiga 520-2194, Japan. 

 

 

 

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Citation: Komatsuzaki N, Aoki-Yoshida A, Shima J (2017). Effects of live Lactobacillus paracasei NFRI 7415 on the intestinal immune system and intestinal microflora of mice. Int. J. Biotechnol. Food Sci. 5(4): 56-62.

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 Abstract 


This study examined the effect of Lactobacillus paracasei NFRI 7415 on the intestinal immune system and intestinal microflora of mice. Two studies were performed. In study 1, ten 5-week-old female BALB/c mice were assigned to a control (CO) group or lactic acid bacteria (LB) group for 4 weeks. The LB group freely ingested water containing L. paracasei NFRI 7415 (106 cfu/ml). After the feeding period, the intestinal bacterial flora in the feces were investigated. The total counts of anaerobic bacteria in the feces were higher in the CO group than in the LB group, although the total counts of aerobic bacteria were not significantly different between the two groups. No harmful bacteria such as Enterococci and Bacteroides spp. were detected in the feces of the LB group. A Basic Local Alignment Search Tool (BLAST) search revealed that one of the anaerobic bacteria in the feces of LB group showed 100% homology to L. paracasei. In study 2, the effects of L. paracasei NFRI 7415 on the gut immune system of BALB/c mice were evaluated in vitro. As a result, production of the cytokine IL-12 in Peyers’ patch (PP) and mesenteric lymph nodes (MLN) cells, which are gut-associated lymphoid tissues (GALT), was increased by treatment with L. paracasei NFRI 7415 in a concentration-dependent manner. These results indicate that oral administration of live L. paracasei NFRI 7415 may have the potential to improve intestinal conditions and immune function in humans.

Keywords  Lactobacillus   intestinal immune system   intestinal microflora   IL-12 




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