Article abstract

International Journal of Biotechnology and Food Science

Research Article | Published September 2017 | Volume 5, Issue 3, pp. 42-47

 

In vitro antifungal activity of Croton macrostachys and Allium sativum extracts against Candida albicans and Trichophyton mentagrophytes isolates

 

 

 

 

 

 Gemechu Hailu1

 Molalegne Bitew2

 Mathewos Temesgen3*

 

  Email Author

 

   1.    Office of Livestock Health, Jarso District, Western Wollega, Ethiopia.

 

   2.    Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia.

 

   3.    Department of Biology, Ambo University, Ethiopia.

 

 

 

 

 

 

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Citation: Hailu G, Bite M, Temesgen M (2017). In vitro antifungal activity of Croton macrostachys and Allium sativum extracts against Candida albicans and Trichophyton mentagrophytes isolates. Int. J. Biotechnol. Food Sci. 5(3): 42-47.

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 Abstract 


In-vitro studies on the efficacy of crude extracts of Croton macrostachys and Allium sativum against Candida albicans and Trichophyton mentagrophytes was conducted in JimmaTown, Ethiopia. The leaves and the bulb cloves of Croton macrostachys and Allium sativum, respectively, were collected and the crude plant extracts were prepared. The in-vitro antifungal activity was evaluated at six different concentrations by agar disc diffusion method for several replicates, and the activity obtained was not concentration dependent except methanolic extract of Croton macrostachys on the strain of mould. The results were compared with standard antifungal drug (Ketoconazole) and distilled water. T. mentagrophytes was more susceptible fungal strain, while Candida albicans was more resistant one. The results revealed that the methanolic extract of A. sativum demonstrated more growth inhibitory activity against both fungi strains which studied than methanolic and aqueous extract of C. macrostachys. There was no significant variation in the zone of inhibition between the different concentrations (p > 0.05). However, there was high significant variation between the plant species, method of extraction and the tested fungus (p < 0.001). Findings from this study confirmed that plant extracts can be used as natural fungicides to control pathogenic fungi, thus reducing the dependence on the synthetic fungicides.

Keywords  Candidia albicans   crude plant extracts   fungi   Trichophyton mentagrophytes   Jimma




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