Article abstract

International Journal of Biotechnology and Food Science

Research Article | Published December 2018 | Volume 6, Issue 5, pp. 86-94

 

In-vitro effect of some plant extracts and synthetic fungicide in the control of cocoyam leaf necrotic fungi in Aguata LGA, Anambra State

 

 

 

 

 

Iwuagwu, C. C.1*

Nwogbaga, A. C.2

Onejeme, F. C.1

Nduka, U. F.1

Iheaturu, D. E.1

 

  Email Author

Tel: +2348061156141.

 

1.    Department of Crop Science and Horticulture, Nnamdi Azikiwe University. Awka Anambra State, Nigeria.

 

2.    Department of Crop Production and Landscape Management Ebonyi State University, Abakaliki, Nigeria.

 

 

 

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Citation: Iwuagwu CC, Nwagbaga AC, Onejeme FC, Nduka UF, Iheaturu DE (2018). In-vitro effect of some plant extracts and synthetic fungicide in the control of cocoyam leaf necrotic fungi in Aguata LGA, Anambra State. Int. J. Biotechnol. Food Sci. 6(5): 86-94.

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 Abstract 


Cocoyam leaves of two tropical crops, taro (old cocoyam, Colocasia esculenta (L.) Schott) and blue or purple taro (new cocoyam tanier, Xanthosoma sagittifolium (L.) Schott) obtained from the farmers’ field in two villages in Ekwulobia of Aguata local government area of Anambra State. The villages included Ula and Okpo. Cocoyam leaves and petioles showing small dark-brown or black lesions were collected for isolation of the causal pathogen. The organism recovered from the infected cocoyam leaves was Aspergillus niger. This organism may have had access into these cocoyam leaves through wounds created by working equipment (for harvesting) and pests. The antifungal effects of ethanol, methanol and petroleum ether extracts of lemon grass leaves and stalks (Cymbopogon citratus) and fruits of pepper fruit (Dennettia tripetala) on the growth of Aspergillus niger were investigated in-vitro at concentrations of 1, 1.5, and 2 g/ml, while the synthetic fungicide (Apron plus) was used as a standard control. The data collected were analyzed using ANOVA. The experimental design used was split – split – plots in completely randomized design with three replications and means were separated using least significant difference (LSD) at 5% probability level. All plant extracts and Apron plus inhibited the fungus. Apron plus caused the highest level of inhibition (75%) followed by lemon grass (49.04%) while the least was pepper fruit (39.48%). The study also showed that the higher the concentration, the higher the inhibition with 2g/ml having the highest inhibition effect (72.58%) for synthetic fungicide The extract of lemon grass (Cymbopogon citratus) had a higher percentage inhibition value in the days of the culture, while Soxhlet extraction using Pet ether as the extracting solvent had the highest inhibition level (53.03%). Thus, the plant extracts used in this research could be suggested as an alternative to synthetic fungicides. More studies should be carried out on these plant extracts to identify their active ingredients to facilitate their commercial production and availability to farmers as these extracts are expected to be biodegradable and may reduce the rate of application of synthetic fungicides which are detrimental to human health and are more persist in the environment.

Keywords  In-vitro experiments   plant extracts   synthetic fungicide   cocoyam   fungi   Anambra State 



 

Copyright © 2018 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0




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