Article abstract

International Journal of Biotechnology and Food Science

Research Article | Published July 2018 | Volume 6, Issue 2, pp. 33-41

 

Effect of some physico-chemical parameters on the production of hydrolytic cell wall degrading enzymes by 2 strains Fusarium oxysporum f. sp. elaeidis

 

 

 

 

 

Ekhorutomwen O. E.1,2*

Samuel T. O.2

Chidi N. I.1,2

Udoh M. E.1

Ogbebor C. O.1

Omoregie K. O.1

 

  Email Author

 

1.   Nigerian Institute for Oil Palm Research (NIFOR), Plant Pathology Division, PMB 1030 Benin City, Edo State, Nigeria.

 

2.    Department of Botany, University of Lagos, Yaba, Lagos State, Nigeria.

    

 

 

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Citation: Ekhorutomwen OE, Samuel TO, Chidi NI, Udoh ME, Ogbebor CO, Omoregie KO (2018). Effect of some physico-chemical parameters on the production of hydrolytic cell wall degrading enzymes by 2 strains Fusarium oxysporum f. sp. elaeidis. Int. J. Biotechnol. Food Sci. 6(2): 18-32.

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 Abstract 


Extracellular endoglucanase, exoglucanase, β-glucosidase, and endo-polymethylgalacturonase enzyme activities were detected in culture filtrates of two pathogenic strains of Fusarium oxysporum f. sp. elaeidis of oil palm. The effect of carbon source, incubation period, pH and temperature were used to detect the production of these enzymes. Although there were no significant differences in the means of enzymes between the two carbon sources used, pectin medium enhanced higher production of endoglucanase and exoglucanase, while β-glucosidase and pectinase show higher production in carboxymethylcellulose medium. On the effect of incubation period on enzymes production, the optimum production of these enzymes was achieved on both 4th and 8th day. Optimum production of enzymes occurred at pH 5.5 except for β-glucosidase which optimum production was pH 7.5. Lastly, optimum temperature for the production of these enzymes was 30°C. Result from this study shows that physico-chemical parameters such as carbon sources, incubation period, pH and temperature influenced the production of these enzymes.

Keywords  Endoglucanase   exoglucanase   β-glucosidase   endo-polymethylgalacturonase   Fusarium oxysporum f. sp. elaeidis 



 

Copyright © 2018 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0




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