Article abstract

International Journal of Biotechnology and Food Science

Research Article | Published March 2018 | Volume 6, Issue 1, pp. 9-17

 

Isolation of amylase and cellulase producing fungi from decaying tubers and optimization of their enzyme production in solid and submerged cultures

 

 

 

 

 

 Christiana N. Ogbonna*

 Onyinyechukwu Blessing Nnaji

 Onyinyechukwu J. Chioke

 

  Email Author

 

 Department of Plant Science and Biotechnology, University of Nigeria, Nsukka, Nigeria.

 

 

 

 

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Citation: Ogbonna CN, Nnaji OB, Chioke OJ (2018). Isolation of amylase and cellulase producing fungi from decaying tubers and optimization of their enzyme production in solid and submerged cultures. Int. J. Biotechnol. Food Sci. 6(1): 9-17.

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 Abstract 


The aim of this work was to isolate hydrolytic enzyme-producing fungi from decaying tubers and to optimize their enzyme production in solid and submerged cultures. Five fungal genera were isolated from decaying cassava, cocoyam, white yam, and sweet potato tubers. Amylase and cellulase production were qualitatively detected in PDA and CMC plates of the isolates using Lugol’s iodine and 1% Congo red staining solution techniques respectively. The five fungal genera were identified as Aspergillus species I and II, Penicillium sp., Rhizopus sp. and Trichoderma sp. based on their macroscopic and microscopic features with reference to Dichotomous Key for fungi. Amylase and cellulase production by the isolates in solid state and submerged cultures were optimized using rice grains, sweet potato, white yam, cassava and cocoyam flours as substrates. Aspergillus species I produced the highest glucoamylase units of 572.16 ± 7.92 and 482.70 ± 2.00 µmole/g of solid substrate from rice grains and sweet potato flour mixed with 20% rice husk respectively. Rhizopus species produced the highest cellulase units of 263.52 ± 3.68 µmole/g from sweet potato flours in solid substrate fermentation. Cellulase, gluco- and alpha-amylase productivities were generally higher in solid state than in submerged cultures. The results of this experiment have shown that decaying tubers are good sources of amylase and cellulase producing fungi. Amylase and cellulase production capacity of the isolates of Aspergillus and Rhizopus species are quite high and they have high potentials for biotechnological applications.

Keywords  Fungi   amylase   cellulase   tubers   fermentation   diseased tubers 



 

Copyright © 2018 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0




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