Article abstract

International Journal of Biotechnology and Food Science

Research Article | Published October 2019 | Volume 7, Issue 4, pp. 56-64.

doi: https://doi.org/10.33495/ijbfs_v7i4.19.108

 

Developing molecular tools capable of identifying, characterizing and distinguishing phytoplasma strains responsible for the emerging lethal yellowing disease (LYD) of coconut in Nigeria

 

 

 

 

 

Ekhorutomwen O. E.1*

Udoh M. E.1

Umaru F. F.2

Aghayedo C. O.3

Iserhienrhien A.4

 

  Email Author

 

1. Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), PMB 1030 Benin City, Edo State, Nigeria.

 

2. Department of Biological Sciences, Taraba State University, Jalingo, Taraba State, Nigeria.

 

3. Entomology Division, Nigerian Institute for Oil Palm Research (NIFOR), PMB 1030 Benin City, Edo State, Nigeria.

 

4. Physiology Division, Nigerian Institute for Oil Palm Research (NIFOR), PMB 1030 Benin City, Edo State, Nigeria.

 

 

 

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Citation: Ekhorutomwen OE, Udoh ME, Umoru FF, Aghayedo CO, Iserhienrhien A (2019). Developing molecular tools capable of identifying, characterizing and distinguishing phytoplasma strains responsible for the emerging lethal yellowing disease (LYD) of coconut in Nigeria. Int. J. Biotechnol. Food Sci. 7(4): 56-64.

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 Abstract 


Lethal yellowing disease (LYD) of coconut palms is caused by a wall-less prokaryote in the genus Ca. Phytoplasma. Phytoplasmas contained different groups and subgroups. Molecular techniques together with symptoms in host plants, host plants species, host plant variety and geographical location are required for identification and classifying phytoplasmas into various group and subgroups. On this basis, the phytoplasma associated with the LYD of coconut belong to the subgroup in the Coconut Lethal Yellows Group. Phytoplasma-specific PCR assays generally utilize primers specific to the conserved regions of the 16SrRNA gene and to the variable 16S-23S intergenic region. Universal primers and group-specific primers have been developed and are used routinely to detect, characterize and distinguish phytoplasma infections in host plants and insect vectors. However, the unreliable results for LYD detection in samples using previously available strain specific primers has necessitated the need to develop new primers that are efficient and reproducible in detecting LYD, including possible new emerging LYD phytoplasma variants. A database of more than 60 phytoplasma spacer region (SR) sequences now exists, which should facilitate the identification of other phytoplasma clade-specific PCR primers. Hence, the need to explore molecular methods and procedures that will enhance the detection and characterization of the phytoplasma strains responsible for the LYD of coconut in Nigeria.

Keywords  LYD   PCR   phytoplasma   primers   spacer region



 

Copyright © 2019 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0




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