Article abstract

Journal of Agricultural and Crop Research

Research Article | Published October 2017 | Volume 5, Issue 4, pp. 66-76

 

Enhancement of maize seeds germination by magnetopriming in perspective with reactive oxygen species

 


 

 

M. B. Shine1

S. Kataria1*

K. N. Guruprasad1

Anjali Anand2

 

Email Author

 

1.     School of Life Sciences, Devi Ahilya University, Khandwa Road, Indore, India.


2.     Plant Physiology, Indian Agricultural Research Institute, Pusa, New Delhi, India.


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 Citation: Shine MB, Kataria S, Guruprasad KN, Anand A (2017). Enhancement of maize seeds germination by magnetopriming in perspective with reactive oxygen species. J. Agric. Crop Res. 5(4): 66-76.

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 Abstract 


The utility of stationary magnetic field (SMF) for biostimulation of seeds and associated physio-biochemical changes in maize (Zea mays L.) var. HQPM.1 was studied under laboratory conditions. Magnetopriming improved germination related parameters like percentage of germination, speed of germination, seedling length, fresh weight, dry weight and vigour indices. Among the various parameters seedling length and vigour Index I were the most improved parameters (up to 72 to 59% respectively) after magnetopriming. Two doses (200 mT for 60 min and 100 mT for 120 min) were further taken to understand the involvement of reactive oxygen species (O2.-, .OH and H2O2) and antioxidant enzymes in the magnetoprimed seeds as they were best in improving germination related parameters. Enhanced germination in magnetoprimed maize seeds was coupled with enhanced reactive oxygen species content. Peroxidase (both cytosolic and wall bound) activity was enhanced and superoxide (both cytosolic and wall bound) activity was reduced in the embryo and the eight-day-old seedlings from magnetoprimed seeds. Obtained results indicated that magnetopriming can be effectively used as a biostimulant for seed germination and the impact of SMF is biochemically identified in context with reactive oxygen species.

Keywords  Biostimulation   magnetopriming   reactive oxygen species   seedling growth 

 

 

 

  References 

 

Abdul-Baki AA, Anderson JD (1973). Vigour determination in soybean by multiple criteria. Crop Sci. 10:31-34.

Aladjadjiyan A (2002). Study of the influence of magnetic field on some biological characteristics of Zea mays. J. Central Eur. Agric. 3:89-94.

Bailly C, El-Maarouf-Bouteau H, Corbineau F (2008). From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. C. R. Biol. 331:806-814.

Beauchamp CO, Fridovich I (1971). Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-278.

Bhardwaj J, Anand A, Nagarajan S (2012). Biochemical and biophysical changes associated with magnetopriming in germinating cucumber seeds. Plant Physiol. Biochem. 57:67-73.

Bhatnagar D, Deb AR (1977). Some aspects of pre-germination exposure of wheat seeds to magnetic fields: Germination and early growth. Seed Res. 5:129-137.

Carpita NC (1984). Cell wall development in maize coleoptiles. Plant Physiol. 76:205-212.

Chance B, Maehly AC (1955). Assay of catalases and peroxidases. Methods in Enzymology11, P.S. Colowick, N.O. Kaplan (Eds.), Academic Press, New York pp. 764-775.

Chen SX, Schopfer P (1999). Hydroxyl-radical production in physiological reactions: a novel function of peroxidase. Eur. J. Biochem. 260:726-735.

EI-Maarouf Bouteau H, Bailly C (2008). Oxidative signaling in seed germination and dormancy. Plant Signal. Behav. 3:175-182.

Florez M, Carbonell MV, Martinez E (2007). Exposure of maize seeds to stationary magnetic fields: Effects on germination and early growth. Environ. Exp. Bot. 59:68-75.

Frahry G, Schopfer P (2001). NADH stimulated, cyanide resistant superoxide production in maize coleoptiles analyzed with tetrazolium based assay. Planta 212:175-183.

Giannopolitis CN, Ries K (1977). Superoxide dismutases. I. Occurrences in higher plants, Plant Physiol. 59:309-314.

Gondim FA, Gomes-Filho E, Lacerda CF, Prisco JT, Azevedo Neto AD and Marques EC (2010). Pre-treatment with H2O2 in maize seeds: effects on germination and seedling acclimation to salt stress. Braz. J. Plant Physiol. 22:103-112.

Gutzeit HO (2001). Biological effects of ELF-EMF enhanced stress response: New insights and new questions. Electromagnetobiol. 20:15-26.

Ishibashi Y, Yamamoto K, Tawaratsumida T, Yuasa T, Iwaya-Inoue M (2008). Hydrogen peroxide scavenging regulates germination ability during wheat (Triticum aestivum L.) seed maturation. Plant Signal. Behav. 3:183-188.

ISTA (1985). International seed testing association. Seed Sci. Technol. 13:299-513.

Jinapang P, Prakob P, Wongwattananard P, Islam NE, Kirawanich P (2010). Growth characteristics of mung beans and water convolvuluses exposed to 425-MHz electromagnetic fields. Bioelectromagn. 31:519-527.

Kataria S, Baghel L, Guruprasad KN (2015). Acceleration of germination and early growth characteristics of soybean and maize after pre‐treatment of seeds with static magnetic field. Int. J. Tropical Agri. 33: 985-992.

Kataria S, Baghel L, Guruprasad KN (2017). Pre-treatment of seeds with static magnetic field improves germination and early growth characteristics under salt stress in maize and soybean. Biocatal. Agric.Biotech.10: 83-90.

Kavi PS (1983). The effect of non-homogeneous, gradient magnetic field on the magnetic susceptibility values of in sit ragi (Eleusine coracana Gaertn) seed material. Mysore J. Agric. Sci. 17:121-123.

Kukavica B, Mojovic M, Vucinic Z, Maksimovic V, Takahama U, Jovanovic SV (2009). Generation of hydroxyl radical in isolated Pea root cell wall, and the role of cell wall-bound peroxidase, Mn-SOD and phenolics in their production. Plant Cell Physiol. 50:304-317.

Lin CC, Kao CH (2001). Cell wall peroxidase activity, hydrogen peroxide level and NaCl-inhibited root growth of rice seedlings. Plant Soil 230:135-143.

Liszkay A, Kenk B, Schopfer P (2003). Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth. Planta. 217: 658-667.

Liszkay A, Van Der Zalm A, Schopfer P (2004). Production of reactive oxygen intermediates (O2.-, H2O2 and .OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 230: 3114-3123.

Liu Y, Ye N, Liu R, Chen M, Zhang J (2010). H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. J. Exp. Bot. 61 (11):2979-2990.

Lowry HO, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurements with Folin reagent. J. Biol. Chem. 193:265-275.

Maguire JD (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigour. Crop Sci. 2:176-177.

Martínez E, Carbonell MV, Flórez M, Amaya JM, Maqueda R (2009). Germination of tomato seeds (Lycopersicon esculentum L.) under magnetic field. Int. Agrophys. 23:45-49.

Mukherjee SP, Choudhuri MA (1983). Implications of water stress-induced changes in the leaves of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol. Plant. 58:166-170.

Naglreiter C, Reichenauer TG, Goodman BA, Bolhar-Nordonkampf HR (2005). Free radical generation in Pinus sylvestris and Larix deciduas seeds primed with polyethylene glycol or potassium salt solution. Plant Physiol. Biotechnol. 43:117-123.

Podlesny J, Misiak L, Koper R (2001). Concentration of free radicals in faba bean seeds after the pre-sowing treatment of the seeds with laser light. Int. Agrophys.15:185-189.

Podlesny J, Misiak LE, Podlesna A, Pietruszewski S (2005). Concentration of free radicals in pea seeds after the pre-sowing treatment of the seeds with magnetic field. Int. Agrophys.19:243-249.

Ramos CL, Pou S, Britigan BE, Cohen MS, Rosen GM (1992). Spin trapping evidence for myeloperoxidase-dependent hydroxyl radical formation by human neutophils and monocytes. J. Biol. Chem. 267: 8307-8312.

Rodriguez AA, Grunberg KA, Taleisnik EL (2002). Reactive oxygen species in the elongation zone of maize leaves are necessary for leaf extension. Plant Physiol. 129:1627-1632.

Schopfer P, Plachy C, Frahry G (2001). Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol. 125:1591-1602.

Schweikert C, Liszkay A, Schopfer P (2000). Scission of polysaccharides peroxidase-generated hydroxyl radicals. Phytochem. 53:565-570.

Shine MB, Guruprasad KN (2012). Impact of pre‐sowing magnetic field exposure of seeds to stationary magnetic field on growth, reactive oxygen species and photosynthesis of maize under field conditions. Acta Physiol. Plant. 34:255-265.

Shine MB, Guruprasad KN, Anand A (2011a). Enhancement of germination, growth, and photosynthesis in soybean by pretreatment of seeds with magnetic field. Bioelectromagn. 32:474-484.

Shine MB, Guruprasad KN, Anand A (2011b). Superoxide radical production and performance index of Photosystem II in leaves from magnetoprimed soybean seeds. Plant Signal. Behav. 6:1636-1638.

Shine MB, Guruprasad KN, Anand A (2012). Effect of stationary magnetic field strengths of 150 and 200 mT on reactive oxygen species production in soybean. Bioelectromagn. 33:428-437.

Thomas S, Anand A, Chinnusamy V, Dahuja A, Basu S (2013). Magneto‐priming circumvents the effect of salinity stress on germination in chickpea seeds. Acta Physiol. Plant. 35:3401-3411.

Vashisth A, Joshi DK (2016). Growth characteristics of maize seeds exposed to magnetic field. Bioelectromagn. DOI: 10.1002/bem.22023.

Vashisth A, Nagarajan S (2008). Exposure of seeds to static magnetic field enhances germination and early growth characteristics in chickpea (Cicer arietinum L). Bioelectromagn. 29:571-578.

Walters C (1998). Understanding the mechanism and kinetics of seed ageing. Seed Sci. Res. 8:223-244.

Weaver JC (1993). Combined environmental exposures to chemicals and transient magnetic fields: A hypothesis for possible human health hazards. Proc. Bioelectromagnetics Society, 16th Annual Meeting, 12–16 June, 1993, Copenhagen, Denmark. p. 36.

Wittekind E, Broers D, Kraepelin G, Lamprecht I (1990). Influence of non-thermic AC magnetic fields on spore germination in a dimorphic fungus. Radiat. Environ. Biophys. 29:143-152.

Yinan L, Yuan L, Yongquing Y, Chunyang L (2005). Effect of seed pre‐treatment by magnetic field on the sensitivity of cucumber (Cucumis sativus) seedlings to ultraviolet-B radiation. Environ. Exp. Bot. 54:286-294.

Yokota K, Yamazaki I (1965). Reaction of peroxidase with reduced nicotinamide-adenine dinuleotide phosphate. Biochimic. Biophysica Acta 105:301-312.

Zepeda-Bautista R, Hernández-Aguilar C, Domínguez-Pacheco A, Cruz-Orea A, Godina-Nava JJ, Martínez-Ortíz E (2010). Electromagnetic field and seed vigour of corn hybrids. Int. Agrophys. 24:329-332.