Article abstract

Journal of Agricultural and Crop Research

Research Article | Published August 2020 | Volume 8, Issue 8 pp. 169-175.

doi: https://doi.org/10.33495/jacr_v8i8.20.140

 

Optimal seed water content and freezing method for cryopreservation of Areca catechu L. seeds

 



 

 

Lin Zeng1

Hong-Qiong Tan1

Ya-Kun Gu1

Jian-He Wei1,2*

 

Email Author

 

1. Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China.

2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.


……....…...………..........................…………....………............…............……...........……........................................................………...……..…....……....…

Citation: Zeng L, Tan H, Gu Y, Wei J (2020). Optimal seed water content and freezing method for cryopreservation of Areca catechu L. seeds. J. Agric. Crop Res. 8(8):169-175. doi: 10.33495/jacr_v8i8.20.140.

……....…...………..........................…………....………............…............……...........……........................................................………...……..…....……....…



 Abstract 


Areca catechu is an important cash crop in tropical regions of Asia and Central Africa. The seed is susceptible to low humidity and temperatures as recalcitrant seeds, so it cannot be stored by conventional methods, only can be sowed soon after collecting. The aim of this study was to explore a long-term effective preservation method with cryopreservation for A. catechu seeds. Mature seeds responses to drying were tested. Further, we analyzed the effects of liquid nitrogen freezing on the microstructure and biochemical indicators of the seeds. Desiccation of seeds with moisture content (MC) from 55 to 20% reduced their germination rate from 90% to 40% in significance. Stored at 25°C and 4°C for 2 months, the germination rates declined with increasing of seed moisture content. The optimum safe MC in A. catechu seeds is 35%. When stored in liquid nitrogen, the vigor of seeds with MC of 35% treated by direct freezing was highest compared to vitrification and step freezing. Direct freezing had no effect on the seed microstructure, but enhanced free radical scavenging activity, and weakened the seed's antioxidant activity. Notably, freezing had no effect on dehydrogenase explaining the high vigor observed. The vitality of seeds with 35% MC after cryopreservation was 95.67%. Liquid nitrogen preserves biochemical indicators and maintains high vitality; therefore, cryopreservation is an effective method for A. catechu seeds.

Keywords  Areca catechu   recalcitrant seed   liquid nitrogen   moisture content  

 

 

Copyright © 2020 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

 

 

  References 

 

Berjak P, Pammenter NW (2014). Cryostorage of germplasm of tropical recalcitrant-seeded species: approaches and problems. Inter J Plant Sci. 175(1):29-39.

Bernardo GG, Oneto C, Antunes P, Rodrigues MF, Empis JM (2001). Extractions from cherry seed oil using supercritical carbon dioxide. Eur. Food Res. Technol. 212:170-174.

Byron Z, Inaudis C, René CR, Lourdes Y, Carlos A, Justo G, Florent E, Marcos EM José CL (2013). Biochemical characterization of Ecuadorian wild Solanum lycopersicum MILL. Plants produced from non-cryopreserved and cryopreserved seeds. CryoLetters. 34(4):413-421.

Chen XL, Zhang JM, Xin X, Huang B, Lu XX (2013). Progress on Cryopreservation State and Research of Plant Germplasm Resources. J. Plant Genet. Resour. 14(3):414-427.

Edilene APF, Ronan CC, Ricardo TF, Roberto JT (2016). Cryopreservation of seeds of Encholirium spectabile Martius ex Schultes by the vitrification method. Rev. Ciênc. Agron. 47(1):172-177.

Elena P, Elena M, Sim HH, Praveen S, Du Hkim (2016). Cryopreservation of Prunus padus L. seeds: emphasizing the significance of Bayesian methods for data analysis. Canad J. For. Res. 46(6):1-33.

Elif AO, Ergun K (2012). Cryopreservation of Thymus cariensis and T. vulgaris Shoot Tips: Comparison of Three Vitrification-Based Methods. CryoLetters. 33(5):363-375.

Florent E (2010). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell. Dev. Biol.-Plant. 47:5-16.

Fu JR, Song SQ (2004). Recalcitrant seed biology. China Science and Culture Press. Beijing, pp. 46-61.

Heloisa OS, Maria LMC, Carla MC, Stefânia VBC, Edila VRVP, João AO (2016). Physiological and biochemical aspects of castor beans seeds deterioration stored in different packaging conditions and temperatures1. J. Seed Sci. 38(3):241-247.

Huang HD (2017). 2015 Areca catechu industry development report and situation forecast. World Trop. Agric. Info. 1:31-39.

Hor YL, Kim YJ, Ugap A, Chabrillange N, Sinniah UR, Engelmann F, Dussert S (2005). Optimal hydration status for cryopreservation of intermediate oil seeds: Citrus as a case study. Ann Bot London. 95:1153-1161.

Liu ZF (2010). Seed science experiment guide. Chemical Industry Press, Beijing. pp. 62-105.

Lu LL, Zhou YK, Gan BC, Zhang QQ (2016). Seed breeding technology regulation of Areca catechu. Hainan Local Standard. DB 46/T 386-2016.

Ma QQ, Xu L, Li ZY, Li KL (2007). Recent developments in cryopreservation of plant germplasm. Chin. J. Trop. Crop. 28(1):105-110.

Marcin M, Beata PPM, Paweł C (2015). A new insight in desiccation tolerance and cryopreservation of mazzard cherry (Prunus avium L.) seeds. Open Life Sci. 10:354-364.

Nan O, Borges M, Konan KJL, Hocher V, Verdeil JL, Tregear J, Engelmann F, Malaurie B (2012). A simple protocol for cryopreservation of zygotic embryos of ten accessions of coconut (Cocos nucifera L.) In Vitro Cell. Dev. Biol. Plant. 48:160-166.

Pammenter NW, Patricia B (2014). Physiology of Desiccation-Sensitive (Recalcitrant) Seeds and the Implications for Cryopreservation. Int. J. Plant Sci. 175(1):21-28.

Pan RZ (2004). Plant physiology. Higher Education Press. Beijing, pp. 283-284. Roberts EH (1973). Predicting the storage life of seeds. Seed Sci. Technol. 1:499-51.

Sakai A, Kobayashi S, Oiyama I (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. Var. brasiliensis Tanaka) by vitrification. Plant Cell Rep. 9(1):30.

Shao YT (2006). Study on Changes in Desiccation Tolerance and Cryopreservation of Archontophoenix alexandrae Seeds during Their Development. University of Chin. Acad. Sci. pp. 39-40.

Shao YT, Yin SH, Lan QY, Zhang XY (2006). Developmental Changes in Relation to Desiccation Tolerance of Archontophoenix alexandrae (Palmae) Seeds. Acta Botanica Yunnanica. 28(5):515-522.

Stanislawa P, Ewelina R (2014). Factors influencing the storability of Fagus sylvatica L. seeds after release from dormancy. Plant Growth Regul. 72:17-27.

Sun L, Song HB, Zhang L, Huang JK, Wang JX, Yang XH, Sun GB, Gao XM, Sun XB (2017). Systematic evaluation for safety of traditional Chinese medicine Areca catechu and its preparations. Chin. J. Chin Mat. Med. 42(21):4067-4073.

Wang MR, Maurizio L, Florent E, Ranjith P, Bart P, Gayle MV, Wang QC (2020). Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources. PCTOC, https://doi.org/10.1007/s11240-020-01770-0.

Wang XK, Huang JL (2015). Principles and techniques of plant physiological and biochemical experiments. Higher Education Press, Beijing. pp. 167-168.

Wen B (2011). Cytological and physiological changes related to cryotolerance in recalcitrant Livistona chinensis embryos during seed development. Protoplasma. 248:483-491.

Wen B, Wang XF, Tan YH, Song SQ (2013). Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation. In Vitro Cell Dev. Biol. Plant. 49:717-723.

Wu Y, Gu YK, Fu L, Zeng L (2019). Myristica fragrans Seeds: Cryopreservation Technology and Physiological and Biochemical Activity. Chin. Agric. Sci. Bullet. 35(19):78-82.

Yang LZ, Liu XX, Li ZP (2018). Research on Production Status and Technology of Areca catechu in the World. World Agri. 07:121-128.

Yin YP, Dong XH (2008). Seed science experiment technique. China Agriculture Press, Beijing. pp. 23-67.

Zeng L, Gu YK, Wu Y, Zheng XL (2018). Effect of Cryopreservation on Structure, Physiology and Biochemical Characteristics of Dipterocarpus turbinatus Seeds. J. Trop. Sub. Bot. 26(3):249-254.

Zeng L, He MJ, Chen K, Wei JH (2014). Cryopreservation study on seeds and embryos in Dalbergia odorifera. Chin J Chin Materia Med. 39(12):2263-2266.

Zhang CJ, Lv FJ, Tao HT (2008). Research progress on activity constituents and function of Areca catechu L. Food Nutr. (China). 6:50-53.

Zhang D, Ren L, Chen GQ, Zhang J, Barbara MR, Shen XH (2015). ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox. Plant Cell Rep. 34:1499-1513.

Zhang HR (1999). Principles and methods of biochemical testing. Ningxia People's Publishing House, Yinchuan. pp. 59-63.

Zhang SZ, Xu PF, Wu JJ (2012). Crop seed physiology experiment. Chemical Industry Press, Beijing. pp. 75-76.

Zhang ZL, Qu WJ (2005). Plant Physiology Experiment Guide, 3rd edn. Higher Education, Beijing. pp. 35-229.

Zhou WH, Li ZH, Zhang HD, Jiang AM, Cui YY (2010). GC-MS Analysis of Fatty Acids of Areca Nut Seed Oil. J. Chin. Cereals Oils Association. 25(8):38-41.

Zhou WJ, Luo JY, Liu HM, Zhao M, Ou YZ, Yang MH (2017). Change of Pharmacological Components of Arecae Semen under Different Storage Conditions. Chin. J. Exper. Trad. Med. Formulae. 23(19):56-64.