Article abstract

Journal of Agricultural and Crop Research

Research Article | Published February 2020 | Volume 8, Issue 2 pp. 20-32.

doi: https://doi.org/10.33495/jacr_v8i2.19.169

 

Evaluation of heat treatment and bio-priming of cowpea seeds for the management of fusarium wilt disease in the screen house

 


 

 

Ayodele M. Ajayi*

Bosede J. Evbenata

 

Email Author
Tel: +2348132158030

 

Department of Crop, Soil and Pest Management. Federal University of Technology, Akure, P. M. B, 704, Akure, Ondo State, Nigeria.


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Citation: Ajayi AM, Evbenata BJ (2020). Evaluation of heat treatment and bio-priming of cowpea seeds for the management of fusarium wilt disease in the screen house. J. Agric. Crop Res. 8(2):20-32.

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 Abstract 


Fusarium wilt is one of the most devastating diseases of cowpea. The pathogen, Fusarium oxysporum f.sp. tracheiphilum is soil and seed-borne. A non-chemical approach was designed for the management of the disease in the present study. Hot water (physical) at three temperatures, 40 50 and 60°C, and Trichoderma viride (biological) at 106 spores/ml were evaluated singly and synergistically in the laboratory. The duration of exposure to hot water was 5 min, after which seeds were allowed to cool followed by priming in sterile water (sole hot water treatment) or T. viride (synergistic physical and bio-control) medium in Petri-dishes for 72 h. The control consisted of seed treated with water at ambient temperature, while the standard check was seeds dressed with Mancozeb fungicide. Both were primed in a sterile water medium. Nine treatments were evaluated in all. Germinated seeds were transplanted into plastic pots containing soil infested with F. oxysporum at 106 spores/ml in the screen house. The experimental layout was completely randomized design and data collected were subjected to statistical analysis and mean separation. Results showed that seeds treated with 50°C hot water only had the highest germination percentage, 96.66%, and seedling vigour, 460.98. Disease incidence and severity values were least in seeds treated with synergistic hot water at 50°C and priming in T. viride medium. The highest number (77.33) and weight (13.41 g) of seeds were also recorded for the same treatment, while the least, 18 and 3.60 g were obtained from control. Integrated use of hot water (50°C) and bio-priming in T. viride medium can be recommended for use in the management of fusarium wilt disease of cowpea.

Keywords  Fusarium wilt    cowpea    hot water   Trichoderma viride   germination   vigour   yield 

 

 

Copyright © 2020 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

 

 

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