Article abstract

Journal of Agricultural and Crop Research

Research Article | Published April 2022 | Volume 10, Issue 1. pp. 14-22.

doi: https://doi.org/10.33495/jacr_v10i1.21.171

 

Antagonistic action of two species of Trichoderma spp. Against Colletotrichum gloeosporioides: The causal agent of cashew anthracnose in Benin

 



 

 

Dénis Ehinnoudé Tonon Houndahouan1,3*

Rachidatou Sikirou1

Ouorou Kobi Douro Kpindou2

Cathérine-Marie Mélanie Roméo Akpodji1

Aristide Adomou3

Valérien Zinsou4

Marie Epiphane Eyinkitin Akinni Dossoumou1

Kouami N’djolosse5

 

Email Author

 

1. Laboratoire de Défense des Cultures (LDC), Centre de Recherches Agricoles d’Agonkanmey, Institut National des Recherches Agricoles du Bénin (INRAB), 01 BP 884 Cotonou, Bénin.

2. International Institute of Tropical Agriculture, Station du Bénin, 08 BP 0932. Cotonou, Bénin.

3. Département de Biologie Végétale, Faculté des Sciences et Techniques, Université d’Abomey-Calavi (UAC), Bénin.

4. Faculté d’Agronomie, Université de Parakou (UP), BP 123 Parakou, Bénin.

5. Centre de Recherche Agricole du Centre Bénin, Institut National des Recherches Agricoles du Bénin (INRAB), 01 BP 884 Cotonou, Bénin.


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Citation: Houndahouan DET, Sikirou R, Kpindou OKD, Akpodji CMR, Adomou A, Zinsou V, Dossoumou MEEA, N’djolosse K (2022). Antagonistic action of two species of Trichoderma spp. Against Colletotrichum gloeosporioides: The causal agent of cashew anthracnose in Benin. J. Agric. Crop Res. 10(1):14-22. doi: 10.33495/jacr_v10i1.21.171.

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 Abstract 


The biological control of plant diseases by the use of antagonist micro-organisms is a strategy used in integrated pest management strategies in agricultural production. The potential of 3 strains of Trichoderma harzianum and 3 strains of T. pseudokoningii as biological control agents against Colletotrichum gloeosporioides was assessed in this study. The trials were conducted in vitro and in vivo with 7 and 26 treatments, respectively, utilising a completely randomly randomized design. Antifungal activity of Trichoderma species was evaluated in vitro against C. gloeosporioides mycelial radial growth and conidial germination. In vivo, disease incidence and severity were assessed on cashew seedlings inoculated with the following modalities: preventive (no presence of pathogen), simultaneous, and curative (in the presence of pathogen) modality. In vitro, T. harzianum and T. pseudokoningii inhibited the growth of C. gloeosporioides by up to 64.31 and 71.41%, and its germination rate by up to 74.38 and 78.78%, respectively. In vivo, therapy with T. harzianum and T. pseudokoningii significantly reduced the incidence and severity of anthracnose (P < 0.0001), regardless of the inoculation modality. T. harzianum reduced the incidence and severity of the disease by more than 92 and 97%, respectively. T. pseudokoningii reduced the disease incidence by up to 85% in curative and simultaneous inoculation modalities, and by up to 96%when used in preventive modality. The three modalities of T. pseudokoningii reduced the disease's severity by up to 95%.These findings imply that T. harzianum or T. pseudokoningii may be suitable for effective biological control of cashew anthracnose.

Keywords  Anacardium occidentale   Biological control   Anthracnose  

 

 

Copyright © 2022 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

 

 

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