Impedimetric evaluation of the synergistic effects of oregano and cranberry aqueous extract mixtures on the growth of Salmonella typhimurium.
Ajayi Adeyinka. O.
Research Article | Published December 2013
Medicinal and Aromatic Plant Research Journal, Vol. 1(2), pp. 12-19
Natural Resources Institute, University of Greenwich at Medway, Chatham Maritime, Kent, United Kingdom. E-mail: checkyinka2010@yahoo.com
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Salmonella is a foodborne, Gram negative and facultative anaerobic bacteria and continues to be a serious public health concern especially in the tropics. Salmonella typhimurium is one of the most economically important serotype of Salmonella and it is the most common non-typhoid serotype isolated from pork and humans. It is estimated that more than 63.1% of S. typhimurium infections in humans are due to pork-associated cases. Oregano and cranberry extracts are attested to have potent antimicrobial properties; however, the effect of an oregano-cranberry extract combination has not been assessed on S. typhimurium. The synergistic efficacy of oregano-cranberry extract mixtures was evaluated against S. typhimurium in broth systems through the use of a RABIT system. Oregano-cranberry extract mixtures of different proportions were added at 0.5 and 1% v/v into RABIT cells containing 50 µl of bacterial cells and then
incubated (30°C). Conductance was recorded automatically at 6 min intervals for 24 h. The impedimetric detection times (TTD) were automatically determined by the RABIT system as indicated when there were three consecutive changes in conductivity greater than 5 µs/6 min. Results obtained showed that the synergies of oregano-cranberry extract combinations produced significant effects (P < 0.05) on the TTD. Oregano-cranberry (50-50% w/w, 1% v/v) treatment was most effective at inhibiting the growth of S. typhimurium with the longest TTD of 9.36 h. In conclusion, synergies of the combined plant extracts were significantly more effective in inhibiting growth of S. typhimurium than were individual extracts of the same plants. The results show a promising potential for the application of the combined extracts in pork systems.
Keywords: Phytochemical synergies, oregano, cranberry, TTD, S. typhimurium.
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Citation: Ajayi AO (2013). AImpedimetric evaluation of the synergistic effects of oregano and cranberry aqueous extract mixtures on the growth of Salmonella typhimurium. Med. Aromat. Plant Res. J. 1(2): 12-19.
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