Efficacy studies on Mist Diodia, a herbal preparation for the management of hypertension in rodents.

 

J. Asiedu-Larbi^1*, J. P. Adjimani², L. K. N. Okine², M. E. Addy², A. K. Nyarko³ and B.J.B. Nyarko⁴

 

Research Article | Published March 2014

Medicinal and Aromatic Plant Research Journal, Vol. 2(1), pp. 6-17

 

 

¹Department of Pharmacology and Toxicology, Centre for Plant Medicine Research, (CPMR) Mampong, Ghana.

²Department of Biochemistry, Cell and Molecular Bology, University of Ghana, Legon, Ghana.

³Chemical Pathology Unit, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.

⁴Ghana Research Reactor-1, Ghana Atomic Energy Commission (GAEC), Kwabenya, Accra, Ghana.

 

*Corresponding author. E-mail: bigbigjerry@yahoo.com.  Tel: +233-0244803662.

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The anti-hypertensive property of Mist Diodia, a herbal preparation for the management of hypertension, was assessed in two murine models of hypertension. Systolic and diastolic blood pressures were recorded using the Tail-cuff method under non-anaesthetized conditions. In vitro tissue chymase enzyme activity assay, serum and urine concentration of sodium and potassium ions and urine volumes were measured, to ascertain the possible involvement of the renin-angiotensin-aldosterone system (RAAS) in the anti-hypertensive activity. Lipid profiles and serum total antioxidant concentration were determined to find out if anti-hypertensive activity was due to improved lipidemia. The results indicated that Mist Diodia possesses significant (p < 0.05) anti-hypertensive activities in the two models used in this present study. Mist Diodia significantly (p < 0.05) increased urine output in all the models used. Sera from animal groups treated with Mist Diodia significantly (p < 0.05) inhibited tissue chymase enzyme activity in vitro. The antioxidant status and lipid profiles were significantly (p < 0.05) enhanced. Serum concentrations of sodium and potassium were significantly (p < 0.05) reduced while the corresponding urine concentrations of these two ions increased in all the animal models. It could therefore be concluded, that Mist Diodia has anti-hypertensive activity which may be mediated through the RAAS with accompanying diuresis. Improvement in lipidemia could be a possible mechanism for its action. These findings thus, support its ethno-medicinal use.

 

Keywords: Mist Diodia, Diodia scandens, Aframomum melegueta, SHRs, L-NAME, anti-hypertensive, SDRs.

 

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Citation: Asiedu-Larbi J, Adjimani JP, Okine LKN, Addy ME, Nyarko AK,Nyarko BJB (2014). Efficacy studies on Mist Diodia, a herbal preparation for the management of hypertension in rodents. Med. Aromat. Plant Res. J. 2(1): 6-17.

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