Inhibitory bioactive metabolites produced by actinomycetes isolated from mangrove ecosystem of Khor Kalba’ shores, UAE
Ismail Saadoun1*, Khafiya Bin Humoudi1, Marim Kalander1, Ban Al-Joubori1 and Ziad W Jaradat2
Research Article | Published August 2013
International Journal of Biotechnology and Food Science, Vol. 1(2), pp. 39-45
1Department of Applied Biology, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirate.
2Fatima College of Health Sciences, Institute of Applied Technology, Al Ain, United Arab Emirate.
*Corresponding author. E-mail: isaadoun@sharjah.ac.ae Tel: +971 6 5053807, Fax: 971 6 5053814.
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In this study, marine sediment habitat of mangrove ecosystem of Khor Kalba’ Shores, Emirate of Sharjah-UAE was investigated for the presence of inhibitory and antiangiogenic compounds-producing actinomycetes. Marine sediments were enriched to recover actinomycetes on starch casein nitrate (SCN) agar medium. The potential of the recovered colonies to inhibit the growth of different Gram positive and negative multi-resistant bacterial pathogens was tested by the agar disc diffusion method. Data showed that all recovered isolates were able to produce inhibitory bioactive compound(s) against one or more of the tested bacterial pathogens with zones of inhibition ranging between 4 and 55 mm in diameter. However, three isolates KM1, KM2 and KM3 showed maximal inhibition against Pseudomonas aeruginosa with 55, 48 and 45 mm diameter zones of inhibition, respectively. It is noted that the isolate KM18 exhibited an inhibitory action against all tested bacterial pathogens except
Klebseilla sp., with zones of inhibition ranging between 18 and 42 mm diameter. After extraction of KM1 metabolites with the solvent butanol:ethylacetate, the concentrated extract showed antiangiogensis activity as reflected by the reduction of the neovascular index from 3.1/cm2 to 0.5/cm2. Partial molecular characterization of the bioactive metabolites-producing actinomycetes by PCR analysis using universal and Actino specific primers for the 16S rRNA gene fragment showed a single 500 and 350 bp band, respectively. Based on the results of this study, it can be concluded that the inhibitory effect of the recovered actinomycetes isolates from unique habitats in Sharjah (UAE) may suggest the novelty of the inhibitory/antiangiogenic compound(s) produced by KM1 strain and/or the other promising strains.
Keywords: Actinomycetes, bioactivity, antiangiogensis, antibacterial, metabolites, PCR.
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Citation: Ismail S, Khafiya BH, Marim K, Ban Al-Joubor, Ziad WJ (2013). Inhibitory bioactive metabolites produced by actinomycetes isolated from mangrove ecosystem of Khor Kalba’ shores, UAE. Int. J. Biotechnol. Food Sci. 1(2): 39-45
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