Phytochemical screening of the antimicrobial fraction of Solanum indicum L. berries extract and evaluation of its effect against the survival of bacteria pathogens of plants

 

Irène Ahou Kouadio^1*, Olivier Kouame Chatigre¹ and Mireille Bretin Dosso²

 

Research Article | Published January 2014

International Journal of Biotechnology and Food Science, Vol. 2(1), pp. 16-20

 

 

¹Laboratory of Biochemistry and Food Sciences, UFR Biosciences, University of Felix Houphouet-Boigny, 22 BP 582 Abidjan 22, Ivory Coast.

²Pasteur Institute of Ivory Coast 01 BP 490 Abidjan 01, Cote d’Ivoire.

 

*Corresponding author. E-mail: irenekouadio@yahoo.fr.  Tel: 00225 07250511. Fax: 00225 22444473.

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In this study, the effect of the antimicrobial fraction of Solanum indicum L. berries extract on growth and the survival of three species of bacteria pathogens of plants (Pseudomonas aeruginosa, Pseudomonas fluorescens and Pseudomonas syringae) was evaluated. The inhibitory activity of thisantimicrobial fraction was observed on the growth of the three species tested. However, only a total inhibition of the growth of Pseudomonas syringae was observed. This antimicrobial fraction possessed also the capacity of causing the death of the cells of this species. Indeed, the percentage in reduction of Alamar blue indicating cell viability which was 100% in the medium without antimicrobial fraction decreased to reach the value of 5.08% in the medium at 2% of antimicrobial fraction for this species after five days of incubation. At this same concentration, only about 50 % of the cells were still alive after five days of incubation for P. aeruginosa and P. fluorescens. The phytochemical screening of the antimicrobial fraction revealed compounds including flavonoids, carotenoids and saponins. The Thin Layer Chromatography analysis on this antimicrobial fraction showed Rf values of 0.28, 0.38 and 0.95. This antimicrobial fraction could thus be used as natural substance to prevent infection of plants and crops by bacteria pathogens and also as preservative in food and feed as these berries have been ingested by humans for quite some time without any toxic effect noted.

 

Keywords: Solanum indicum L., berries, bacteria species, surviving cells, antimicrobial fraction.

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Citation: Irène AK, Olivier KC, Mireille BD (2014). Phytochemical screening of the antimicrobial fraction of Solanum indicum L. berries extract and evaluation of its effect against the survival of bacteria pathogens of plants. Int. J. Biotechnol. Food Sci. 2(1): 16-20

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