PCR and ELISA detection of cassava mosaic virus in a Congolese cassava landrace
Otono Freddy Bulubulu1*, Ndofunsu Aimé Diamuini1, Nakweti Ruffin Kikakedimau1, Ntumbula Alexandre Mbaya1, Hity Mutambel1, Kasali Lumande2, Ndiku Luyindula1, Gladys Rufflard,3
and Philippe Lepoivre3.
Research Article | Published February 2015
International Journal of Biotechnology and Food Science, Vol. 3(1), pp. 10-16
1Département de Biotechnologie et Génétique moléculaire, Commissariat Général à l’Energie Atomique (CGEA/CREN-K.), B.P. 868 Kin XI Kinshasa/République Démocratique du Congo (RDC).
2Faculté des Sciences, Université de Kinshasa, B.P. 190 KinXI, RDC.
3Laboratoire de Phytopathologie, Gembloux Agro-BioTech, Université de Liège, Passage des Déportés 2, Gembloux 5030, Belgique..
*Corresponding author. E-mail: freddy.bulubulu@upn.ac.cd, freddybbl@yahoo.fr.
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The aim of this study is to diagnosing the cassava mosaic virus in the cuttings of the Congolese cassava landrace Manihot esculenta var. Boma. Symptoms severity was evaluated by visual observation of leaves in field and in greenhouse. The PCR (polymerase chain reaction) and DAS-ELISA tests were used to detect the cassava mosaic virus in this variety. The results showed that the disease index on infected plants were about 3.3 in the field and 2.8 in the greenhouse respectively. The presence of African Cassava Mosaic Virus (ACMV) in samples of this variety was detected by DAS-ELISA. The polymerase chain reaction was well performed for the identification of ACMV and not successful for the Uganda variant of East African Mosaic Virus (EACMV-UG) identification in the leaves samples with or without symptoms.
Keywords: Cassava, virus identification, severity, molecular and serology detection.
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Citation: Bulubulu OF, Diamuini NA, Kikakedimau NR, Mbaya NA, Mutambel H, Lumande K, Luyindula N, Rufflard G, Lepoivre P (2015). PCR and ELISA detection of cassava mosaic virus in a Congolese cassava landrace. Int. J. Biotechnol. Food Sci. 3(1): 10-16.
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